Determination of mercury (II) by spectrophotometric method based on self assembly of gold nanoparticles


College of Chemistry and Environmental Science, Hebei University, Baoding 071002, P.R.. China


Due to the binding of the –COO- group with the mercury (II), gold nanoparticles
functionalized with glutathione can self-assemble to form a supermolecular network in the HAc-
NaAc buffer solution. The aggregation of functionalized gold nanoparticles and mercury (II)
caused the shift of maximum absorption peak of the UV-Vis spectrum from 520 nm to 609 nm.
Based on this principle, a simple spectrophotometric method for determination of mercury (II)
was preliminarily established. The detection limit of the developed method for determination of
mercury (II) was about 8.5×10-8 mol L-1.


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